Protein Details: P2X purinoceptor 1

Protein ID

ICDB_Pro_0537

Protein Name

P2X purinoceptor 1

Gene Name

P2rx1

Organism

Rattus norvegicus (Rat)

Length

399 amino acids

AlphaFoldDB

AF-P47824-F1-model_v4.pdb

Function

ATP-gated nonselective transmembrane cation channel permeable to potassium; sodium and with relatively high calcium permeability. Furthermore; CTP functions as a weak affinity agonist for P2RX1 (By similarity). Plays a role a role in urogenital; immune and cardiovascular function (By similarity). Specifically; plays an important role in neurogenic contraction of smooth muscle of the vas deferens; and therefore is essential for normal male reproductive function (By similarity). In addition; contributes to smooth muscle contractions of the urinary bladder (By similarity). On platelets; contributes to platelet activation and aggregation and thereby; also to thrombosis (By similarity). On neutrophils; it is involved in chemotaxis and in mitigating the activation of circulating cells (By similarity).

Sequence

MARRLQDELSAFFFEYDTPRMVLVRNKKVGVIFRLIQLVVLVYVIGWVFVYEKGYQTSSDLISSVSVKLKGLAVTQLQGLGPQVWDVADYVFPAHGDSSFVVMTNFIVTPQQTQGHCAENPEGGICQDDSGCTPGKAERKAQGIRTGNCVPFNGTVKTCEIFGWCPVEVDDKIPSPALLREAENFTLFIKNSISFPRFKVNRRNLVEEVNGTYMKKCLYHKIQHPLCPVFNLGYVVRESGQDFRSLAEKGGVVGITIDWKCDLDWHVRHCKPIYQFHGLYGEKNLSPGFNFRFARHFVQNGTNRRHLFKVFGIHFDILVDGKAGKFDIIPTMTTIGSGIGIFGVATVLCDLLLLHILPKRHYYKQKKFKYAEDMGPGEGEHDPVATSSTLGLQENMRTS

PDB Structures

Ligand Binding

1. DICL_CP

2. DICL_Pep

Binding Site

BINDING 68; /ligand="CTP"; /ligand_id="ChEBI:CHEBI:37563"; /evidence="ECO:0000250|UniProtKB:F8W463"; BINDING 70; /ligand="ATP"; /ligand_id="ChEBI:CHEBI:30616"; /evidence="ECO:0000250|UniProtKB:P56373"; BINDING 70; /ligand="CTP"; /ligand_id="ChEBI:CHEBI:37563"; /evidence="ECO:0000250|UniProtKB:F8W463"; BINDING 140; /ligand="CTP"; /ligand_id="ChEBI:CHEBI:37563"; /evidence="ECO:0000250|UniProtKB:F8W463"; BINDING 186; /ligand="ATP"; /ligand_id="ChEBI:CHEBI:30616"; /evidence="ECO:0000250|UniProtKB:P56373"; BINDING 186; /ligand="CTP"; /ligand_id="ChEBI:CHEBI:37563"; /evidence="ECO:0000250|UniProtKB:F8W463"; BINDING 286; /ligand="ATP"; /ligand_id="ChEBI:CHEBI:30616"; /evidence="ECO:0000250|UniProtKB:P56373"; BINDING 290; /ligand="ATP"; /ligand_id="ChEBI:CHEBI:30616"; /evidence="ECO:0000250|UniProtKB:P56373"; BINDING 290; /ligand="CTP"; /ligand_id="ChEBI:CHEBI:37563"; /evidence="ECO:0000250|UniProtKB:F8W463"; BINDING 292; /ligand="ATP"; /ligand_id="ChEBI:CHEBI:30616"; /evidence="ECO:0000250|UniProtKB:P56373"; BINDING 292; /ligand="CTP"; /ligand_id="ChEBI:CHEBI:37563"; /evidence="ECO:0000250|UniProtKB:F8W463"; BINDING 309; /ligand="ATP"; /ligand_id="ChEBI:CHEBI:30616"; /evidence="ECO:0000250|UniProtKB:P56373"; BINDING 309; /ligand="CTP"; /ligand_id="ChEBI:CHEBI:37563"; /evidence="ECO:0000250|UniProtKB:F8W463"

Disease

Location

High levels in vas deferens and urinary bladder. Lower extent in spinal cord; coeliac ganglion; lung and spleen (probably in the smooth muscle part of both organs).

DOI ID

10.1038/371516a0; 10.1101/gr.2596504; 10.1128/mcb.11.8.4177-4188.1991; 10.1093/emboj/17.11.3016; 10.1124/mol.54.6.989; 10.1016/j.jmb.2004.06.092; 10.1111/j.1471-4159.2004.02939.x

RefSeq

NP_001135839.1; NP_001272344.1; NP_037129.1;